Tantalus said:
http://www.igenex.com/lymeset1.htm
Lyme Antibody Serology
Lyme IgG/IgM Antibody Serology
The Lyme IgG/IgM Antibody Serology test is an enzyme linked immunoassay (ELISA) which indicates the presence of IgG and IgM antibodies to B. burgdorferi. The IgG antibody often persists long after symptoms have disappeared. The presence of antibody indicates exposure, not necessarily active disease. A positive or equivocal result must be confirmed by both IgG and IgM Western Blots.
Lyme IgM Antibody Serology
The Lyme IgM Antibody assay is another serologic test in ELISA format, and it detects the presence of IgM antibodies after exposure to an infected tick. IgM antibodies apppear early in response to infection, and this test may be positive two to six weeks following exposure. Because of the test's lack of specificity, a positive or equivocal result must be confirmed by an IgM Western Blot.
Lyme Western Blot
IgG Western Blot
The IgG Western Blot is a sandwich-type immunoassay performed in a manner that allows the antibody response to be visualized. It is a qualitative test and is generally more sensitive and specific than the ELISA.
IgM Western Blot
This test is a very sensitive indicator of exposure to B. burgdorferi. It may be positive as early as one week after a tick bite, and will usually remain positive for six to eight weeks after initial exposure. A positive IgM result with clinical history can indicate early Lyme disease, or even persistent infection in otherwise serologically negative individuals.
Antigen Detection
Lyme Dot Blot Assay (LDA)*
The LDA is an immunoassay for the direct detection of Lyme antigen in urine that reacts specifically to rabbit anti-B. burgdorferi antibodies. The rabbit antibodies are specifically targeted to the following B. burgdorferi antigens: 23kDA25kDA (Osp C), 31 kDA (Osp A), 34 kDA (Osp B), 39kDA and/or 93kDA. If the Borrelia specific antigen(s) are present in the urine sample, a dot with a bluish-purple precipitate forms on the membrane. The limit of detection in urine spiked with sonicated B. burgdorferi is 12.5 ng/ml of urine.
Reverse Western Blot (RWB) for Antigen*
The RWB is an immunoassay for direct detection and identification of Lyme antigen in urine which specifically react with rabbit anti-B. burgdorferi antibodies. The rabbit antibodies are specifically targeted to the following B. burgdorferi antigens: 18kDA, 23kDA25kDA (Osp C), 28kDa, 30kDA, 31kDA (Osp A), 34kDA (Osp B), 39kDA, 45kDA, 58kDA, 66kDA and 93kDA. If any of the Borrelia specific antigen(s) are present in the urine sample, bluish-purple bands are visualized on a membrane.
Keith.